1. Basic hydrobiological and ecological concepts . 2. Hydrobiology of stagnant and running water, self-purification. 3. Trophic classifications of surface water. 4. Saprobic system of surface water quality assessment. 5. Biology of underground water. 6. Biological indicators of pollution. 7. Basic ecotoxicological concepts, kinds of toxicity, factors affecting toxicity. 8. Main pollutants in environment. 9. Effects of xenobiotics on organisms. 10. Sampling and sample preparation, factors affecting results. 11. Ecotoxicological biotests. 12. Evaluation of toxicity tests, interpretation of results. 13. Legislation. Audiovisual presentation of ecotoxicological biotests.

1. Safety training and laboratory regulations. Field and experimental records, measurement protocol. 2. Microscopy - sample preparation and observation. Description of observe in a protocol. 3. Ecotoxicological biotest –Sinapis alba. 4. Ecotoxicological biotest – Lemna minor. 5. The methodology of collection of water and sediment samples. 6. Anthropogenic impact on stagnant and running water.